Crystalline Entity Video Script

INTRODUCTION Hello, and welcome to Crystalline Entity’s design studio, where the name of the game is crystallization. Like a mosaic tile, crystals begin with a single building block that grows into a patterned masterpiece. Patterned crystals of biomolecules, like protein and DNA, can be analyzed by X-ray crystallography. Here, a beam of X-rays shoots a […]

Experimental

Crystallization Protein-DNA Complex In order to achieve a crystalline structure, we must combine the protein and matching DNA to form a Protein-DNA Complex. This process involves finding the best ratio of protein to DNA to add together and incubating the mix. Screening With the DNA bound by the protein, we can now set up crystallization […]

Crystallization

Protein-DNA Complex In order to achieve a crystalline structure, we must combine the protein and matching DNA to form a Protein-DNA Complex. This process involves finding the best ratio of protein to DNA to add together and incubating the mix.   Screening With the DNA bound by the protein, we can now set up crystallization […]

DNA

If DNA is to be crystalized, it is required to be extremely pure. For CSU BIOMOD’s tile designs, HPLC purified DNA was ordered from IDT to achieve said purity. IDT is Integrated DNA Technology which is a company that can synthesize DNA. Here is an in depth paper showing ways DNA can be synthesized. Each […]

Protein

  Cloning A very common method of cloning uses restriction enzymes. Restriction enzymes were first discovered by Ronald W. Davis from Stanford. The basic mechanism is shown in the image below. The complete plasmid will be transformed into E. Coli via heat shock. The E Coli. without the plasmid does not grow. We use a […]

Design

  The Video Script The starting point of our project is to reengineer DNA-protein complexes. What you’re looking at here is an atomic detail model of one of our designable co-crystals: it contains a short double stranded DNA duplex with two monomers of the protein. To prevent confusion, we will use a simplified representation. Now, […]