Blog

DNA

Purification For crystallography, we need DNA that is ultra-pure. When DNA is ordered from a synthesis company, there is a certain level of purity obtained. We can order the DNA very pure but the price sky rockets. Instead, our experimental team collaborated with Dr. Shing Ho, a DNA crystallography lab at CSU, to purify the… Continue reading DNA

Protein

  Cloning A very common method of cloning uses restriction enzymes. Restriction enzymes were first discovered by Ronald W. Davis from Stanford. The basic mechanism is shown in the image below.     We use a version of sequence and ligation independent cloning (SLIC). We call this LICKI...(for you Pokemon fans, this is named after… Continue reading Protein

Design

  The Video Script The starting point of our project is to reengineer DNA-protein complexes. What you’re looking at here is an atomic detail model of one of our designable co-crystals: it contains a short double stranded DNA duplex with two monomers of the protein. To prevent confusion, we will use a simplified representation. Now,… Continue reading Design

The Basics

Here, we will introduce key definitions to help you understand our design. Scientists have observed that short pieces of double stranded DNA spontaneously order end-to-end (Nakata et. al Science 2007).We can use this interaction to build a biomolecular scaffold. The starting materials for this project are co-crystals, made of two or more distinct components. We are specifically using co-crystals… Continue reading The Basics

Applications

The versatility of the DNA inserts will provide a rapidly evolvable material for multiple applications. Current structure determination technology is labor intensive and haphazard. Guest capture is one key use for ICC bioscaffolds. Any guest molecule that binds the designed crystals tightly and specifically may join the periodic lattice that is visible in the electron… Continue reading Applications